Journal: Cell reports

Article Title: Genomic Resolution of DLX-Orchestrated Transcriptional Circuits Driving Development of Forebrain GABAergic Neurons

doi: 10.1016/j.celrep.2019.07.022

Figure Lengend Snippet: (A) Schematic model of DLX genomic function in developing GE. (B) Curated gene regulatory network (GRN) for DLX-regulated transcription factors and lineage specification factors. The GRN is organized radially with regard to the laminar activity of genes (VZ/SVZ, SVZ, and MZ, shown in three different shades of gray). The effects of the Dlx1/2 −/− mutation are indicated in three nested circles. The outer circle reports RNA changes measured by ISH (IS) (this study; , ). The middle circle reports histone PTM signal (H) changes. The inner circle reports RNA-seq (R) changes. Red and green represent repressive and activating roles for DLX TFs on each assay, respectively. For histone changes, REs assigned to nearest TSS.

Article Snippet: Briefly, RNA sequencing libraries were generated using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Mouse, according to the manufacturer’s protocol (Illumina, San Diego, CA, USA).

Techniques: Activity Assay, Mutagenesis, RNA Sequencing

Journal: Cell reports

Article Title: Genomic Resolution of DLX-Orchestrated Transcriptional Circuits Driving Development of Forebrain GABAergic Neurons

doi: 10.1016/j.celrep.2019.07.022

Figure Lengend Snippet:

Article Snippet: Briefly, RNA sequencing libraries were generated using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Mouse, according to the manufacturer’s protocol (Illumina, San Diego, CA, USA).

Techniques: Recombinant, Labeling, Blocking Assay, SYBR Green Assay, Transfection, Protease Inhibitor, Multiplex Assay, Agarose Gel Electrophoresis, Library Quantification, Luciferase, Reporter Assay, Plasmid Preparation, Transgenic Assay, Software